Thymopoietin/LAP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87822

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Thymopoietin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSE

Marker

Inner-nuclear Membrane Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thymopoietin/LAP2 Antibody - BSA Free

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822]

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822]

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunocytochemistry/Immunofluorescence: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human skeletal muscle shows moderate positivity in nuclear membrane in a small subset of myocytes.
Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822]

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822]

Western Blot: Thymopoietin/LAP2 Antibody [NBP1-87822] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-TMPO antibody. Remaining relative intensity is presented.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human lymph node shows strong positivity in nuclear membrane in germinal center cells.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human prostate shows strong positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining of human small intestine shows strong positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP1-87822] - Staining in human lymph node and skeletal muscle tissues using NBP1-87822 antibody. Corresponding TMPO RNA-seq data are presented for the same tissues.

Applications for Thymopoietin/LAP2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Thymopoietin

Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth, LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.

Alternate Names

TMPO

Gene Symbol

TMPO

Additional Thymopoietin Products

Product Documents for Thymopoietin/LAP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Thymopoietin/LAP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Thymopoietin/LAP2 Antibody - BSA Free

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Protocols

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