Thymopoietin/LAP2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-03478

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Thymopoietin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-410 of human Thymopoietin/LAP2 (NP_001027454.1). MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISASCRRPIKGAAGRPLELSDFRMEESFSSKYVPKYVPLADVKSEKTKKGRS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Thymopoietin/LAP2 Antibody - Azide and BSA Free

Western Blot: Thymopoietin/LAP2 AntibodyAzide and BSA Free [NBP3-03478]

Western Blot: Thymopoietin/LAP2 AntibodyAzide and BSA Free [NBP3-03478]

Western Blot: Thymopoietin/LAP2 Antibody [NBP3-03478] - Western blot analysis of extracts of HeLa cells, using Thymopoietin/LAP2 antibody (NBP3-03478) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody - Azide and BSA Free [NBP3-03478]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody - Azide and BSA Free [NBP3-03478]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP3-03478] - Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] Thymopoietin/LAP2 Rabbit pAb (NBP3-03478) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody - Azide and BSA Free [NBP3-03478]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody - Azide and BSA Free [NBP3-03478]

Immunohistochemistry-Paraffin: Thymopoietin/LAP2 Antibody [NBP3-03478] - Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] Thymopoietin/LAP2 Rabbit pAb (NBP3-03478) at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Thymopoietin/LAP2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] -

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] - Immunofluorescence analysis of MCF7 cells using Thymopoietin/LAP2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Thymopoietin/LAP2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] -

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] - Immunofluorescence analysis of HepG2 cells using Thymopoietin/LAP2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Thymopoietin/LAP2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] -

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] - Immunofluorescence analysis of NIH/3T3 cells using Thymopoietin/LAP2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Thymopoietin/LAP2 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] -

Immunocytochemistry/ Immunofluorescence: Thymopoietin/LAP2 Antibody - Azide and BSA Free [Thymopoietin/LAP2] - Immunofluorescence analysis of HeLa cells using Thymopoietin/LAP2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Thymopoietin/LAP2 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Thymopoietin

Lamins are type V intermediate filament proteins and are grouped into constitutively expressed B-type lamins and developmentally regulated A-type lamins. Lamin-binding proteins in the nuclear lamina and the nuclear interior include several protein families and/or types of proteins in higher eukaryotes such as the inner nuclear membrane proteins, lamin B receptor, emerin, and MANI, three isoforms of lamina-associated polypeptide 1 (LAP 1), and several isoforms of LAP 2. Up to six LAP 2 isoforms derive from a single gene by alternative splicing in mammals and various isoforms have been described in Xenopus. The best characterized LAP2 isoforms are the inner nuclear membrane protein LAP 2 beta and the nucleoplasmic protein LAP 2 alpha, which are identical in their N-terminal 187-amino acid constant region but differ in their C termini. While LAP 2 beta binds to B-type lamins at the nuclear periphery and was suggested to regulate nuclear lamina growth, LAP 2 alpha specifically interacts with A-type lamins within the nuclear interior as part of a detergent/salt-resistant nucleoskeletal structure.

Alternate Names

TMPO

Gene Symbol

TMPO

Additional Thymopoietin Products

Product Documents for Thymopoietin/LAP2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Thymopoietin/LAP2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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