Tight Junction Protein 1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-21051
![Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051]](https://resources.rndsystems.com/images/products/Tight-Junction-Protein-1-Antibody-Western-Blot-NBP2-21051-img0005.jpg "Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051]")
Key Product Details
Validated by
Species Reactivity
Validated:
Predicted:
Applications
Label
Antibody Source
Format
Product Specifications
Immunogen
Localization
Marker
Clonality
Host
Isotype
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Tight Junction Protein 1 Antibody - BSA Free
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051]
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] -
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Tight Junction Protein 1 antibody [N2C1], Internal (NBP2-21051) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (NBP2-21051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (NBP2-21051) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Tight Junction Protein 1 Antibody [NBP2-21051] -
ZO-1 antibody [N2C1], Internal detects TJP1 protein at junction by confocal immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: TJP1 protein stained by ZO-1 antibody [N2C1], Internal (NBP2-21051) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Western Blot: Tight Junction Protein 1 Antibody [NBP2-21051] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N2C1], Internal (NBP2-21051) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Applications for Tight Junction Protein 1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Format
Preservative
Concentration
Shipping
Stability & Storage
Background: Tight Junction Protein 1
Alternate Names
Gene Symbol
UniProt
Additional Tight Junction Protein 1 Products
Product Documents for Tight Junction Protein 1 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Tight Junction Protein 1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Customer Reviews for Tight Junction Protein 1 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Tight Junction Protein 1 Antibody - BSA Free
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Q: Can this antibody be used for rat sample by WB and ICC/IF. It does not mention in the product datasheet.
A: Our Tight Junction Protein 1 antibody with catalogue number NBP2-21051 has been validated for Western blotting and ICC/IF with samples from human, and the antibody is predicted to react with the mouse protein - mouse is covered by our 100% quality guarantee. This antibody has not yet been tested with samples from rat.
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Q: The Molecular Weight of it should be 187KD,why it seems nearly 250KDa in our image. Can you so kind to help him and me?
A: Our Tight Junction Protein 1 antibody with catalogue number NBP2-21051 was raised to a recombinant protein fragment corresponding to a region within amino acids 556 and 939 of ZO-1 (Uniprot ID #Q07157). According to the Uniprot listing, this protein has a theoretical molecular weight of 195kDa, but undergoes multiple post-translational modifications which would increase the weight further. Since this protein is very large, it will not run exactly at the listed molecular weight, however we are confident that NBP2-21051 is detecting a band which corresponds to Tight Junction Protein 1.
-
Q: Can this antibody be used for rat sample by WB and ICC/IF. It does not mention in the product datasheet.
A: Our Tight Junction Protein 1 antibody with catalogue number NBP2-21051 has been validated for Western blotting and ICC/IF with samples from human, and the antibody is predicted to react with the mouse protein - mouse is covered by our 100% quality guarantee. This antibody has not yet been tested with samples from rat.
-
Q: The Molecular Weight of it should be 187KD,why it seems nearly 250KDa in our image. Can you so kind to help him and me?
A: Our Tight Junction Protein 1 antibody with catalogue number NBP2-21051 was raised to a recombinant protein fragment corresponding to a region within amino acids 556 and 939 of ZO-1 (Uniprot ID #Q07157). According to the Uniprot listing, this protein has a theoretical molecular weight of 195kDa, but undergoes multiple post-translational modifications which would increase the weight further. Since this protein is very large, it will not run exactly at the listed molecular weight, however we are confident that NBP2-21051 is detecting a band which corresponds to Tight Junction Protein 1.