Timeless Antibody

Novus Biologicals | Catalog # NB100-40853

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Timeless Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1170 and the C-terminus (residue 1208) of human Timeless using the numbering given in entry NP_003911.1 (GeneID 8914).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Timeless Antibody

Western Blot: Timeless Antibody [NB100-40853]

Western Blot: Timeless Antibody [NB100-40853]

Western Blot: Timeless Antibody [NB100-40853] - Detection of human and mouse Timeless by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry: Timeless Antibody [NB100-40853]

Immunohistochemistry: Timeless Antibody [NB100-40853]

Immunohistochemistry: Timeless Antibody [NB100-40853] - Detection of human Timeless by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless (NB100-40853). Detection: DAB
Western Blot: Timeless Antibody [NB100-40853]

Western Blot: Timeless Antibody [NB100-40853]

Western Blot: Timeless Antibody [NB100-40853] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Timeless antibody NB100-40853 used for IP at 6 ug per reaction. Timeless was also immunoprecipitated by rabbit anti-Timeless antibody NB100-40852. For blotting immunoprecipitated Timeless, NB100-40853 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: Timeless Antibody [NB100-40853]

Western Blot: Timeless Antibody [NB100-40853]

Western Blot: Timeless Antibody [NB100-40853] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-Timeless antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescencewith an exposure time of 75 seconds.
Immunohistochemistry-Paraffin: Timeless Antibody [NB100-40853]

Immunohistochemistry-Paraffin: Timeless Antibody [NB100-40853]

Immunohistochemistry-Paraffin: Timeless Antibody [NB100-40853] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Timeless used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB

Applications for Timeless Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:250

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB100-40853 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Timeless

Timeless plays a role in DNA damage checkpoint responses. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein (referenced from entrez gene).

Alternate Names

FLJ12640, hTIMTIMELESS1, TIM1protein timeless homolog, timeless (Drosophila) homolog, timeless homolog (Drosophila), TIMFLJ20714

Entrez Gene IDs

8914 (Human)

Gene Symbol

TIMELESS

UniProt

Q9UNS1 (Human)

Additional Timeless Products

Product Documents for Timeless Antibody

Certificate of Analysis

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Product Specific Notices for Timeless Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Timeless Antibody

Customer Reviews for Timeless Antibody (1)

4 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23359676
    Species: Human
    Verified Customer | Posted 12/12/2014

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