TIMP-1 Antibody (2A5) - BSA Free

Novus Biologicals | Catalog # NBP3-30888

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rhesus Macaque

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2A5

Format

BSA Free
View all TIMP-1 Primary Antibodies »

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human TIMP-1 protein and mouse myeloma Ag8563 cells. Sequence common in monkey

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse TIMP-1 Antibody (2A5) - BSA Free (NBP3-30888) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TIMP-1 Antibody (2A5) - BSA Free

TIMP-1 Antibody (2A5)

Immunohistochemistry: TIMP-1 Antibody (2A5) [NBP3-30888] -

Immunohistochemistry: TIMP-1 Antibody (2A5) [NBP3-30888] - Left: Immunohistochemical staining of human colon carcinoma tissue using TIMP-1 antibody (Cat. No. NBP3-30888).Right: Western blot using TIMP-1 antibody against recombinant human TIMP-1 proenzyme (400 ng/lane).

Applications for TIMP-1 Antibody (2A5) - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

: 1-5 ug/ml

Western Blot

: 1-2 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Phosphate buffered saline

Format

BSA Free

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIMP-1

Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Gene Symbol

TIMP1

Additional TIMP-1 Products

Product Documents for TIMP-1 Antibody (2A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for TIMP-1 Antibody (2A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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