TIMP-1 Antibody (F31 P2 A5)

Immunocytochemistry/ Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]

Immunocytochemistry/Immunofluorescence: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Analysis of TIMP1 (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a TIMP1 Monclonal Antibody (F31 P2 A5) at a dilution of 1:100 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.

Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551]

Immunohistochemistry-Paraffin: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] - Cancer biopsies of deparaffinized Human breast carcinoma tissue at a dilution of 1:20.

Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] -

TGF-beta 1 induces the transformation from OF to MFB. (A) Western blot for TGF-beta 1 concentration and time; (B) Src and Acta2 expression in SRC gene knockdown group by q-PCR; and (C) the expression levels of alpha -SMA, Col-1, and Timp-1 in the SRC gene knockdown group by Western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32457885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] -

Signaling inhibitors. (A) Acta2 expression after Signaling inhibitors by q-PCR; (B) the expression of Smad3, Nfkb1, Pik3r1, and Akt1 in the SRC gene knockdown group by q-PCR; (C) the expression of alpha -SMA, Col-1, and Timp-1 after Signaling inhibitors by western blot; (D) the phosphorylation of SMAD2/3, NF-kappa B p65 and PI3K p85 proteins in SRC gene knockdown group by western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32457885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: TIMP-1 Antibody (F31 P2 A5) [NB100-74551] -

TGF-beta 1 induces the transformation from OF to MFB. (A) Western blot for TGF-beta 1 concentration and time; (B) Src and Acta2 expression in SRC gene knockdown group by q-PCR; and (C) the expression levels of alpha -SMA, Col-1, and Timp-1 in the SRC gene knockdown group by Western blot. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32457885), licensed under a CC-BY license. Not internally tested by Novus Biologicals.