TIMP-1 Antibody (SR1425) - BSA Free

Novus Biologicals | Catalog # NBP3-21705

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1425 expressed in HEK293

Format

BSA Free
View all TIMP-1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human TIMP-1 (Uniprot #: P01033)

Specificity

Detects endogenous levels of total TIMP-1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TIMP-1 Antibody (SR1425) - BSA Free (NBP3-21705) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TIMP-1 Antibody (SR1425) - BSA Free

Western Blot: TIMP-1 Antibody (SR1425) [NBP3-21705] -

Western Blot: TIMP-1 Antibody (SR1425) [NBP3-21705] - Western blot analysis using NBP3-21705 in HL60 cell lysate treated with TPA.

Applications for TIMP-1 Antibody (SR1425) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TIMP-1

Tissue inhibitor of metalloproteinases 1 (TIMP1) and Tissue inhibitor of metalloproteinases 2 (TIMP 2) have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP1 forms a complex of 1:1 stoichiometry with activated interstitial collagenases, activated stromelysin, active form of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A), and latent and active forms 92kDa Type IV collagenase (also known as MMP0 or gelatinase B). TIMPs inhibit the proteolytic invasiveness of tumour cells and normal placental trophoblast cells. TIMP1 is produced in low (pg/ml) levels by most cell types. Treatment of cells with the phorbol ester TPA stimulates production of TIMP1 in some cell types, but the low protein levels produced often require concentration of cell culture media to visualize the bands by Western Blotting.

Long Name

Tissue Inhibitors of Metalloproteinases 1

Alternate Names

TIMP1

Gene Symbol

TIMP1

Additional TIMP-1 Products

Product Documents for TIMP-1 Antibody (SR1425) - BSA Free

Certificate of Analysis

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Product Specific Notices for TIMP-1 Antibody (SR1425) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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