TIMP-3 Antibody - IHC-Prediluted
Novus Biologicals | Catalog # NBP2-48469
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine, Equine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
IHC-Prediluted
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Product Specifications
Immunogen
TIMP-3 (aa175-211) (Uniprot: P35625)
Reactivity Notes
The amino acid sequence used as the immunogen for the TIMP3 antibody is 100% homologous in human, cow, dog and horse, and 94% homologous in mouse and rat. Sheep data from customer review.
Localization
Cytoplasmic
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for TIMP-3 Antibody - IHC-Prediluted
![Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]](https://beta-resources.rndsystems.com/images/products/TIMP-3-Antibody---IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48469-img0002.jpg "Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]")
Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]
Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469] - TIMP-3 is expressed in first trimester human decidual cells. This image was submitted via customer Review.![Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]](https://beta-resources.rndsystems.com/images/products/TIMP-3-Antibody---IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48469-img0001.jpg "Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]")
Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469]
Immunohistochemistry-Paraffin: TIMP-3 Antibody - IHC-Prediluted [NBP2-48469] - Formalin-fixed, paraffin-embedded human placenta stained with TIMP-3 Rabbit PAb.Applications for TIMP-3 Antibody - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TIMP-3
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
Additional TIMP-3 Products
Product Documents for TIMP-3 Antibody - IHC-Prediluted
Product Specific Notices for TIMP-3 Antibody - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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