Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Equine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

TIMP-3 (aa175-211) (Uniprot: P35625)

Reactivity Notes

The amino acid sequence used as the immunogen for the TIMP3 antibody is 100% homologous in human, cow, dog and horse, and 94% homologous in mouse and rat. Sheep data from customer review.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33098)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TIMP-3 Antibody

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478] - TIMP-3 is expressed in first trimester human decidual cells. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478] - Formalin-fixedn, paraffin-embedded human placenta stained with TIMP3 Rabbit polyclonal antibody

Applications for TIMP-3 Antibody

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-29478 in the following applications:

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TIMP-3

TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Alternate Names

TIMP3

Gene Symbol

TIMP3

UniProt

Additional TIMP-3 Products

Product Documents for TIMP-3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TIMP-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TIMP-3 Antibody (3)

3.7 out of 5
3 Customer Ratings
5 Stars
0%
4 Stars
67%
3 Stars
33%
2 Stars
0%
1 Stars
0%

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Customer Images


Showing  1 - 3 of 3 reviews Showing All
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  • TIMP-3 Antibody
    Name: Shinford Sun
    Application: Western Blot
    Sample Tested: human lung cancer, human lung cancer cell line, fibroblasts, fibroblast cells and Human lung cancer tissue samples
    Species: Human
    Verified Customer | Posted 08/11/2023
    TIMP3 was generally not expressed at a high density and was expected to be present in specific clusters. Was it limited to fibroblasts only? Further validation with this marker is needed across additional cell lines.
    TIMP-3 Antibody NBP2-29478
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • TIMP-3 Antibody
    Name: Ruhul Choudhury
    Application: Immunohistochemistry-Paraffin
    Sample Tested: First trimester human decidua
    Species: Human
    Verified Customer | Posted 01/18/2017
    TIMP-3 is expressed in first trimester human decidual cells.
    Sodium Citrate antigen retrieval
    TIMP-3 Antibody NBP2-29478
  • Name: Bryan Tinsley
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Sheep lymph node
    Species: Other
    Verified Customer | Posted 09/09/2014
    Sheep lymph node using Timp3 antibody
    TIMP-3 Antibody NBP2-29478

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Protocols

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