TLR2 Antibody (T2.5)

Name: TLR2 Antibody (T2.5)
Brand: Novus Biologicals
SKU: NBP2-30097
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-30097

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Citations (4)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface), Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # T2.5

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Product Specifications

Immunogen

This antibody was raised against the extracellular domain of mouse TLR2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for TLR2 Antibody (T2.5)

Flow (Cell Surface): TLR2 Antibody (T2.5) [NBP2-30097]

Flow (Cell Surface): TLR2 Antibody (T2.5) [NBP2-30097] - Analysis using the FITC conjugate of NBP2-30097. Staining of TLR2 in 10^6 mouse bone marrow cells using 0.5 ug of NBP2-30096, 0.25 ug of anti-mouse CD11b, and 0.5 ug of isotype control.

Flow Cytometry: TLR2 Antibody (T2.5) [NBP2-30097]

Flow Cytometry: TLR2 Antibody (T2.5) [NBP2-30097] - Analysis using the FITC conjugate of NBP2-30097. Surface staining of stable HEK293/hTLR2 cells (IML-202, red) and vector control cells (IML-200, green) using TLR2 antibody at 1 ug/10^6 cells.

Western Blot: TLR2 Antibody (T2.5) [NBP2-30097] -

Embryonic ENS culture neurons express TLR2/4/9. a Agarose gel showing specific products of real-time PCR for the assayed genes in ENS culture (PC); water was used as a no-template control (H2O) and rat colon cDNA as positive control (+C). b TLR relative expression in ENS culture in basal conditions (n = 8). c Localisation of TLR in ENS culture ganglia. B-tubulin III was used instead of HuC/D as neuronal marker for colocalisation with TLR9 due to antibody detection incompatibilities. White arrows point to neuronal somas. Scale bars: 25 μm Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27538577), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TLR2 Antibody (T2.5)

Application

Recommended Usage

Flow Cytometry

0.25 - 1.0ug 10^6 cells

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Application Notes

Use in Flow-cell surface reported in scientific literature (PMID 19091080)

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TLR2

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes (4).

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (T2.5)

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Product Specific Notices for TLR2 Antibody (T2.5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.