Key Product Details

Species Reactivity

Human

Applications

Western Blot, Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # TL2.1

Format

Azide and BSA Free
View all TLR2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising BALB/c mice with CHO cells transfected with human TLR2 cDNA.

Specificity

This antibody is specific to TLR2 (extracellular domain) and does not crossreact with human TLR1 or TLR4. TLR2 is involved in the immunological response against bacterial lipoproteins and other microbial cell wall components.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Novus Biologicals Rabbit TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free (NBP3-12062) is a recombinant monoclonal antibody validated for use in WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free [NBP3-12062]

Immunocytochemistry/ Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free [NBP3-12062]

Immunocytochemistry/Immunofluorescence: TLR2 Antibody (TL2.1) - Chimeric [NBP3-12062] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of TL2.1 (NBP3-12062) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12062, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: TLR2

Toll-like receptors are type I transmembrane signaling receptors which are critical for the innate host defense to pathogens. Toll-like receptor 2 (TLR2), known as CD282, has been identified as a receptor that is central to the innate immune system's response to lipoproteins of Gram-negative bacteria and Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. The TL2.1 antibody is useful for blocking studies. It has been reported to block TLR2 agonist-induced cellular activation.

Long Name

Toll-like Receptor 2

Alternate Names

CD282

Gene Symbol

TLR2

Additional TLR2 Products

Product Documents for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TLR2 Antibody (TL2.1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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