Human TLR9 ELISA Kit (Chemiluminescence)

Novus Biologicals | Catalog # NBP2-76638

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL
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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Species

Human

Specificity

This kit recognizes Human TLR9/CD289 in samples. No significant cross-reactivity or interference between Human TLR9/CD289 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 10.63

Inter-Assay Precision (Precision between assays) CV% 9.34

Recovery for Human TLR9 ELISA Kit (Chemiluminescence)

Recovery

93-117%

Linearity

Human TLR9 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human TLR9 ELISA Kit (Chemiluminescence)

Human TLR9 ELISA Kit (Chemiluminescence)

ELISA: Human TLR9 ELISA Kit (Chemiluminescence) [NBP2-76638] -

ELISA: Human TLR9 ELISA Kit (Chemiluminescence) [NBP2-76638] - Standard Curve Reference

Kit Contents for Human TLR9 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TLR9

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.

Long Name

Toll-like Receptor 9

Alternate Names

CD289

Gene Symbol

TLR9

Additional TLR9 Products

Product Documents for Human TLR9 ELISA Kit (Chemiluminescence)

Certificate of Analysis

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Product Specific Notices for Human TLR9 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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