TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74537

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1F9

Format

Azide and BSA Free
View all TMEFF2/Tomoregulin-2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human TMEFF2(NP_057276) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

41.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free

Western Blot: TMEFF2/Tomoregulin-2 Antibody (OTI1F9)Azide and BSA Free [NBP2-74537]

Western Blot: TMEFF2/Tomoregulin-2 Antibody (OTI1F9)Azide and BSA Free [NBP2-74537]

Western Blot: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMEFF2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMEFF2.
Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - Staining of paraffin-embedded Human lymphoma tissue using anti-TMEFF2 mouse monoclonal antibody.
Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TMEFF2 mouse monoclonal antibody.
Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TMEFF2 mouse monoclonal antibody.
Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - Staining of paraffin-embedded Human breast tissue using anti-TMEFF2 mouse monoclonal antibody.
Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - Staining of paraffin-embedded Human Kidney tissue using anti-TMEFF2 mouse monoclonal antibody.
Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537]

Immunohistochemistry: TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free [NBP2-74537] - Staining of paraffin-embedded Human tonsil using anti-TMEFF2 mouse monoclonal antibody.

Applications for TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TMEFF2/Tomoregulin-2

TMEFF2(transmembrane protein with EGF-like and two follistatin-like domains 2), a gene encoding a plasma membrane protein with two follistatin-like domains and one epidermal growth factor-like domain, had limited normal tissue distribution and was highly overexpressed in prostate cancer. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation. Down-regulated in tumor cell lines in response to a high level of methylation in the 5' region. Clone J4B6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TMEFF2 protein.

Long Name

Transmembrane Protein with EFG-like and two Follistatin-like Domains

Alternate Names

TENB2, Tomoregulin-2, Tomoregulin2

Gene Symbol

TMEFF2

Additional TMEFF2/Tomoregulin-2 Products

Product Documents for TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TMEFF2/Tomoregulin-2 Antibody (OTI1F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
EGF Family

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Protocols

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