TMEM119 Antibody - BSA Free

2.6 out of 5

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• (5)

• TMEM119 Antibody

  Name: Anonymous

  Application: Immunocytochemistry

  Sample Tested: Human microglia

  Species: Human

  Verified Customer | Posted 05/20/2021

  Tmem staining with dapi in human microglai cell line

  

• TMEM119 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Frozen

  Sample Tested: Coronal rat brain slices and Adult brain

  Species: Rat

  Verified Customer | Posted 07/28/2020

  Confocal images from the rat hippocampal CA1 area. Green: Tmem119 1:100 + a-rabbit A488 1:200. Red: NT640/660 1:250. Tmem119 labelled most cell bodies in the hippocampus, esp. neuronal somas. Thus, the antibody is not selective for microglial cells.

  Rats were perfused with 4% paraformaldehyde in PBS (pH=7.6). The brain was removed and immersed in PFA then in 30% sucrose. The brain was sliced with a freezing microtome. A mixture of 1% Triton-X and 10% donkey serum (in TRIS) was used for antigen retrieval and blocking. Tmem119 antibody was used in 1:100 dilution and was visualized by an Alexa-488 conjugated anti-rabbit secondary antibody (1:200). Cell bodies were stained with NeuroTrace 640/660 (1:250).

  
  Bio-Techne Response

  Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

• TMEM119 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Frozen

  Sample Tested: Brain (hypothalamus) tissue

  Species: Sheep

  Verified Customer | Posted 03/13/2020

  Photomicrograph at 40x magnification of a sheep hypothalamic section stained using Rabbit polyclonal TMEM119 antibody NBP2-30551. Results showed no positive staining in our tissue using this antibody.

  To test, we used free-floating sheep hypothalamic brain sections from two animals. We did dilutions of 1:100 to 1:1000 and did not see any staining in our tissue using this antibody NBP2-30551. We also employed an antigen retrieval step in one of our runs, and it did not yield any positive staining.

  
  Bio-Techne Response

  This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

• TMEM119 Antibody

  Name: Vicki Swier

  Application: Immunohistochemistry-Paraffin

  Sample Tested: Brain (cerebral cortex), Brain (hippocampus) tissue, brain (thalamus) and brain (aqueduct)

  Species: Swine

  Verified Customer | Posted 04/23/2018

  Pig cerebral cortex

  1:100 dilution. 5 minute DAB stain. Polymer secondary. pH 6 Citrate buffer used for antigen retrieval by steam heat.

  

• TMEM119 Antibody

  Name: Anonymous

  Application: Immunohistochemistry-Frozen

  Sample Tested: optic nerve and Nerve pia mater

  Species: Feline

  Verified Customer | Posted 01/31/2017

  1:200 concentration. Green-TMEM119, Blue-DAPI

  PFA fixed and cryoprotected tissue.

  

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