CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that is constitutively expressed on naive T and NK cells. The interaction of CD28H with B7-H7 induces effector and memory T cell proliferation and activation. CD28H also regulates epithelial cell morphology, focal adhesion contact formation, and cell migration as well as angiogenesis. It interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1.
TMIGD2 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-81164
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CQVDQATAWERLRVKWTKDGAILCQPYITNGSLSLGVCGPQGRLSWQAPSHLTLQLDPVSLNHSGAYVCWAAVEIPELEEAEGNITRLFVDPDDPTQNRNRIA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit TMIGD2 Antibody - BSA Free (NBP1-81164) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for TMIGD2 Antibody - BSA Free
Western Blot: TMIGD2 Antibody [NBP1-81164] -
Western Blot: TMIGD2 Antibody [NBP1-81164] - Analysis in human lymph node tissue.
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -Staining of human gallbladder shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -Staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] - Staining of human cerebellum shows strong membranous/ cytoplasmic positivity in cells in granular layer.
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -Staining of human kidney shows strong membranous positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -
Immunohistochemistry-Paraffin: TMIGD2 Antibody [NBP1-81164] -Staining of human liver shows no positivity in hepatocytes as expected.Applications for TMIGD2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TMIGD2/CD28H
Long Name
Transmembrane And Immunoglobulin Domain Containing 2
Alternate Names
CD28H, IGPR-1, IGPR1
Entrez Gene IDs
126259 (Human)
Gene Symbol
TMIGD2
UniProt
Additional TMIGD2/CD28H Products
Product Documents for TMIGD2 Antibody - BSA Free
Product Specific Notices for TMIGD2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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