TOMM20 Antibody (1B2) - BSA Free

Novus Biologicals | Catalog # NBP2-60248

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B2

Format

BSA Free
View all TOMM20 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Tomm20 (25-145aa) purified from E. coli

Reactivity Notes

Mouse reactivity reported from a verified customer review.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TOMM20 Antibody (1B2) - BSA Free

Western Blot: TOMM20 Antibody (1B2)BSA Free [NBP2-60248]

Western Blot: TOMM20 Antibody (1B2)BSA Free [NBP2-60248]

Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysate Lane 3.: A431 cell lysate Lane 4.: K562 cell lysate Lane 5.: A549 cell lysate Lane 6.: 293T cell lysate Lane 7.: MCF7 cell lysate Lane 8.: SK-OV-3 cell lysate Lane 9.: PC3 cell lysate
Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]

Immunocytochemistry/ Immunofluorescence: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]

Immunocytochemistry/Immunofluorescence: TOMM20 Antibody (1B2) [NBP2-60248] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Flow Cytometry: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]

Flow Cytometry: TOMM20 Antibody (1B2) - BSA Free [NBP2-60248]

Flow Cytometry: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of Tomm20 in HeLa cells. The cell was stained, at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)
TOMM20 Antibody (1B2) - BSA Free

Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] -

Western Blot: TOMM20 Antibody (1B2) [NBP2-60248] - Analysis of mouse bone marrow derived macrophages using TOMM20 at 1:1000 dilution. A single band observed around the 15kDa ladder. The image is captured using chemiluminescence imaging system. Image from verified customer review.

Applications for TOMM20 Antibody (1B2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

2-5 ug

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 5 using NBP2-60248 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOMM20

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore

Long Name

TOMM20

Alternate Names

MAS20, mitochondrial 20 kDa outer membrane protein, mitochondrial import receptor subunit TOM20 homolo, MOM19, outer mitochondrial membrane receptor Tom20, TOM20, translocase of outer mitochondrial membrane 20 hom

Gene Symbol

TOMM20

Additional TOMM20 Products

Product Documents for TOMM20 Antibody (1B2) - BSA Free

Certificate of Analysis

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Product Specific Notices for TOMM20 Antibody (1B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TOMM20 Antibody (1B2) - BSA Free (1)

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Customer Images

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  • TOMM20 Antibody (1B2) - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Bone Marrrow Derived Macrophages
    Species: Mouse
    Verified Customer | Posted 05/29/2023
    A single band can be observed around the 15kDa ladder. The image is captured using chemiluminescence imaging system. The primary antibody dilution used in study is 1:1000.
    TOMM20 Antibody (1B2) - BSA Free NBP2-60248
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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Protocols

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FAQs

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