TOP2A Antibody (TOP2A/1362) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-54545

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Protein Array, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # TOP2A/1362

Format

Azide and BSA Free
View all TOP2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human TOP2A (around aa1352-1493) (exact sequence is proprietary) (Uniprot: P11388)

Localization

Nuclear

Marker

Proliferation & Drug-Resistance Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53282).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TOP2A Antibody (TOP2A/1362) - Azide and BSA Free

Western Blot: TOP2A Antibody (TOP2A/1362)Azide and BSA Free [NBP2-54545]

Western Blot: TOP2A Antibody (TOP2A/1362)Azide and BSA Free [NBP2-54545]

Western Blot: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Western Blot Analysis of human HeLa cell lysate using TOP2A Antibody (TOP2A/1362).
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Bladder Carcinoma stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545]

Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Formalin-fixed, paraffin-embedded Human Tonsil stained with Topoisomerase II alpha Monoclonal Antibody (TOP2A/1362).
Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545]

Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545]

Protein Array: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOP2A Antibody (TOP2A/1362). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.
TOP2A Antibody (TOP2A/1362) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] -

Immunocytochemistry/ Immunofluorescence: TOP2A Antibody (TOP2A/1362) - Azide and BSA Free [NBP2-54545] -

Confocal Immunofluorescence image of HeLa cells using TOP2A Antibody (TOP2A/1362) - Azide and BSA Free. Green (CF488) and Phalloidin (Red) is used to label the nuclei.

Applications for TOP2A Antibody (TOP2A/1362) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml

Western Blot

0.5 - 1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TOP2A

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Gene Symbol

TOP2A

Additional TOP2A Products

Product Documents for TOP2A Antibody (TOP2A/1362) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TOP2A Antibody (TOP2A/1362) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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