TOP2A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86928

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TOP2A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPDETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TOP2A Antibody - BSA Free

Western Blot: TOP2A Antibody [NBP1-86928]

Western Blot: TOP2A Antibody [NBP1-86928]

Western Blot: TOP2A Antibody [NBP1-86928] - MDA-MB-231 and MCF-7 whole cell lysates were loaded (40 ug/lane). 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody at 1:1000, 4C, overnight. WB image submitted by a verified customer review.
TOP2A Antibody - BSA Free Western Blot: TOP2A Antibody - BSA Free [NBP1-86928]

Western Blot: TOP2A Antibody - BSA Free [NBP1-86928]

Analysis in human cell line RH-30.
TOP2A Antibody - BSA Free Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Analysis in human testis and liver tissues Corresponding TOP2A RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928]

Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928]

Immunohistochemistry-Paraffin: TOP2A Antibody [NBP1-86928] - Staining of human testis shows high expression.
TOP2A Antibody - BSA Free Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Staining of human liver shows no positivity in hepatocytes as expected.
TOP2A Antibody - BSA Free Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Staining of human colon shows strong nuclear positivity in glandular cells.
TOP2A Antibody - BSA Free Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Immunohistochemistry-Paraffin: TOP2A Antibody - BSA Free [NBP1-86928]

Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Applications for TOP2A Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/mL
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 5 using NBP1-86928 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TOP2A

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Gene Symbol

TOP2A

Additional TOP2A Products

Product Documents for TOP2A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TOP2A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for TOP2A Antibody - BSA Free

Customer Reviews for TOP2A Antibody - BSA Free (1)

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Customer Images

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  • TOP2A Antibody
    Name: Yongkang Yang
    Application: Western Blot
    Sample Tested: BREAST CANCER CELL LINES, Sample Amount: 40 ug
    Species: Human
    Verified Customer | Posted 03/06/2021
    Western Blot: MDA-MB-231 and MCF-7 whole cell lysates were loaded with 40 ug/lane. 10% SDS-PAGE. TOP2A Antibody (NBP1-86928) primary antibody: 1:1000, 4℃, overnight.  
    TOP2A Antibody - BSA Free NBP1-86928

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Protocols

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FAQs

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