Topoisomerase I Antibody (6D8) - BSA Free

Novus Biologicals | Catalog # NBP3-26278

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6D8 expressed in HEK293

Format

BSA Free
View all Topoisomerase I Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Topoisomerase I [UniProt P11387]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Topoisomerase I Antibody (6D8) - BSA Free

Topoisomerase I Antibody (6D8)

Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Immunoprecipitation: Topoisomerase I Antibody (6D8) [NBP3-26278] - Immunoprecipitating Topoisomerase I in K562 whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26278 in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26278 (2ug) + K562 whole cell lysate (500ug).
Lane 3: K562 whole cell lysate (10ug).
Topoisomerase I Antibody (6D8)

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Topoisomerase I Antibody (6D8)

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Immunohistochemistry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Image of Topoisomerase I Antibody (6D8) diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Topoisomerase I Antibody (6D8)

Western Blot: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Western Blot: Topoisomerase I Antibody (6D8) [NBP3-26278] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate, PC-3 whole cell lysate.
All lanes: Topoisomerase I Antibody at 1: 2000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 91 kDa
Observed band size: 91 kDa
Topoisomerase I Antibody (6D8)

Flow Cytometry: Topoisomerase I Antibody (6D8) [NBP3-26278] -

Flow Cytometry: Topoisomerase I Antibody (6D8) [NBP3-26278] - Overlay histogram showing HepG2 cells stained with Topoisomerase I Antibody (6D8) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Topoisomerase I Antibody (6D8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Topoisomerase I

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Long Name

DNA topoisomerase 1

Alternate Names

DNA topoisomerase I, EC 5.6.2.1, TOP1

Gene Symbol

TOP1

Additional Topoisomerase I Products

Product Documents for Topoisomerase I Antibody (6D8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Topoisomerase I Antibody (6D8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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