TPT1/TCTP Antibody (9H7S4)

Name: TPT1/TCTP Antibody (9H7S4)
Brand: Novus Biologicals
SKU: NBP3-16122
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16122

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9H7S4 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TPT1/TCTP (P13693). MIIYRDLISHDEMFSDIYKIREIADGLCLEVEGKMVSRTEGNIDDSLIGGNASAEGPEGEGTESTVITGVDIVMNHHLQETSFTKEAYKKYIKDYMKSIK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TPT1/TCTP Antibody (9H7S4)

Western Blot: TPT1/TCTP Antibody (9H7S4) [NBP3-16122]

Western Blot: TPT1/TCTP Antibody (9H7S4) [NBP3-16122] - Western blot analysis of extracts of various cell lines, using TPT1/TCTP Rabbit mAb (NBP3-16122) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody (9H7S4) [NBP3-16122]

Immunohistochemistry-Paraffin: TPT1/TCTP Antibody (9H7S4) [NBP3-16122] - Immunohistochemistry of paraffin-embedded rat brain using TPT1/TCTP Rabbit mAb (NBP3-16122) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunoprecipitation: TPT1/TCTP Antibody (9H7S4) [NBP3-16122]

Immunoprecipitation: TPT1/TCTP Antibody (9H7S4) [NBP3-16122] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug TPT1/TCTP antibody (NBP3-16122). Western blot was performed from the immunoprecipitate using TPT1/TCTP antibody (NBP3-16122) at a dilition of 1:1000.

Applications for TPT1/TCTP Antibody (9H7S4)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TPT1/TCTP

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Long Name

Translationally-controlled Tumor Protein 1

Alternate Names

Fortilin, HRF, p02, TCTP

Gene Symbol

TPT1

Additional TPT1/TCTP Products

Product Documents for TPT1/TCTP Antibody (9H7S4)

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Certificate of Analysis

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Product Specific Notices for TPT1/TCTP Antibody (9H7S4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Calcium-binding Proteins and Related Molecules

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