TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80986
![Western Blot: TRA-1-81 Antibody (TRA-1-81)Azide and BSA Free [NBP2-80986]](https://resources.rndsystems.com/images/products/TRA-1-81-Antibody-TRA-1-81-Azide-and-BSA-Free-Western-Blot-NBP2-80986-img0001.jpg "Western Blot: TRA-1-81 Antibody (TRA-1-81)Azide and BSA Free [NBP2-80986]")
Key Product Details
Species Reactivity
Human, Mouse (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # TRA-1-81
Format
Azide and BSA Free
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Product Specifications
Immunogen
Human embryonal carcinoma cell line 2102Ep.
Reactivity Notes
This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.
Localization
Cell Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Scientific Data Images for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free
Western Blot: TRA-1-81 Antibody (TRA-1-81)Azide and BSA Free [NBP2-80986]
Western Blot: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - Analysis of NB100-1833 expression in NTERA-2 cl.D1 whole cell lysate. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]](https://resources.rndsystems.com/images/products/TRA-1-81-Antibody-TRA-1-81-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80986-img0002.jpg "Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]")
Immunocytochemistry/ Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]
Immunocytochemistry/Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Monoclonal Antibody (Catalog # NB100-1833) at 10 ug/mL for 3 hours at room temperature. Cells were stained using an Anti-Mouse IgM Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. Image from the standard format of this antibody.![Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]](https://resources.rndsystems.com/images/products/TRA-1-81-Antibody-TRA-1-81-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80986-img0003.jpg "Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]")
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986]
Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free [NBP2-80986] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-1833. Staining of human cancer line PSN1. Image from verified customer review.Applications for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000. Use reported in scientific literature
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry
1:50-1:200
Immunohistochemistry-Frozen
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1:10-1:500
Western Blot
1:500
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
IgM purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TRA-1-81
Long Name
Tumor-related Antigen-1-81
Alternate Names
TRA181
Additional TRA-1-81 Products
Product Documents for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TRA-1-81 Antibody (TRA-1-81) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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