TRAF-1 Antibody

Novus Biologicals | Catalog # NB100-2822

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRAF-1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KLQSPKHAYVKDD corresponding to C-Terminus according to NP_005649.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRAF-1 Antibody

Western Blot: TRAF-1 Antibody [NB100-2822]

Western Blot: TRAF-1 Antibody [NB100-2822]

Western Blot: TRAF-1 Antibody [NB100-2822] - Analysis of TRAF-1 using NB100-2822 at 0.5ug/ml in U937 cell lysate (35ug protein in RIPA buffer).
Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822]

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822]

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822]

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822]

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for TRAF-1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.3 - 10 ug/ml
Application Notes
WB: Approx. 50-55 kDa band observed in lysates of monocytic leukemia cell line U937 (PMID: 10657935).
IHC: Paraffin embedded Human Thymus and Small Intestine.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAF-1

The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors.

Long Name

TNF Receptor-Associated Factor 1

Alternate Names

EBI6, TRAF1

Entrez Gene IDs

7185 (Human); 22029 (Mouse)

Gene Symbol

TRAF1

UniProt

NP_005649.1 (Human)

Additional TRAF-1 Products

Product Documents for TRAF-1 Antibody

Certificate of Analysis

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Product Specific Notices for TRAF-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAF-1 Antibody

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Protocols

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