TRAF-2 Antibody (3S2A8)

Novus Biologicals | Catalog # NBP3-15682

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3S2A8 expressed in HEK293
View all TRAF-2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-350 of human TRAF-2 (NP_066961.2). DGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-2 Antibody (3S2A8)

TRAF-2 Antibody (3S2A8)

Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] -

Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of lysates from wild type(WT) and TRAF-2 knockdown (KD) 293T cells, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
TRAF-2 Antibody (3S2A8)

Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] -

Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of various lysates, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
TRAF-2 Antibody (3S2A8)

Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] -

Western Blot: TRAF-2 Antibody (3S2A8) [TRAF-2] - Western blot analysis of various lysates, using [KD Validated] TRAF-2 Rabbit mAb at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
TRAF-2 Antibody (3S2A8)

Immunoprecipitation: TRAF-2 Antibody (3S2A8) [TRAF-2] -

Immunoprecipitation: TRAF-2 Antibody (3S2A8) [TRAF-2] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug TRAF-2 antibody. Western blot was performed from the immunoprecipitate using TRAF-2 antibody at a dilution of 1:50000.

Applications for TRAF-2 Antibody (3S2A8)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

1:50 - 1:200

Western Blot

1:10000 - 1:60000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAF-2

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. TRAF2 is typically described as a 501 amino acid (aa) protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed.

Long Name

TNF Receptor-Associated Factor 2

Alternate Names

TRAF2, TRAP3

Gene Symbol

TRAF2

Additional TRAF-2 Products

Product Documents for TRAF-2 Antibody (3S2A8)

Certificate of Analysis

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Product Specific Notices for TRAF-2 Antibody (3S2A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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