TRAF-2 Antibody (9A5) - BSA Free

Novus Biologicals | Catalog # NBP3-26271

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9A5 expressed in HEK293

Format

BSA Free
View all TRAF-2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human TRAF-2 [UniProt Q12933]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-2 Antibody (9A5) - BSA Free

TRAF-2 Antibody (9A5)

Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] -

Immunoprecipitation: TRAF-2 Antibody (9A5) [NBP3-26271] - Immunoprecipitating TRAF-2 in Hela whole cell lysate.
Lane 1: Rabbit control IgG instead of NBP3-26271 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26271 (2ug) + Hela whole cell lysate (500ug).
Lane 3: Hela whole cell lysate (10ug).
TRAF-2 Antibody (9A5)

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] -

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TRAF-2 Antibody (9A5)

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] -

Immunohistochemistry: TRAF-2 Antibody (9A5) [NBP3-26271] - Image of TRAF-2 Antibody (9A5) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
TRAF-2 Antibody (9A5)

Western Blot: TRAF-2 Antibody (9A5) [NBP3-26271] -

Western Blot: TRAF-2 Antibody (9A5) [NBP3-26271] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, K562 whole cell lysate.
All lanes: TRAF-2 Antibody at 1: 1500
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 56, 62, 55, 54 kDa
Observed band size: 56 kDa
TRAF-2 Antibody (9A5)

Flow Cytometry: TRAF-2 Antibody (9A5) [NBP3-26271] -

Flow Cytometry: TRAF-2 Antibody (9A5) [NBP3-26271] - Overlay histogram showing Hela cells stained with TRAF-2 Antibody (9A5) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for TRAF-2 Antibody (9A5) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:20-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: TRAF-2

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. TRAF2 is typically described as a 501 amino acid (aa) protein, GenBank no. NP_066961.2. However, various TRAF2 isoforms have been described. Therefore the molecular weight of TRAF2 detected by Western blot may vary depending on isoforms expressed.

Long Name

TNF Receptor-Associated Factor 2

Alternate Names

TRAF2, TRAP3

Gene Symbol

TRAF2

Additional TRAF-2 Products

Product Documents for TRAF-2 Antibody (9A5) - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAF-2 Antibody (9A5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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