TRAF-3 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56176

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAF-3 Primary Antibodies »

Product Specifications

Immunogen

A recombinant protein fragment corresponding to the cytoplasmic end of human TRAF3 (amino acids 341-568) was used as immunogen, GenBank no. GenBank no. gi|22027616|ref|NP_003291.2|.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-3 Antibody - BSA Free

Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56176]

Immunohistochemistry-Paraffin: TRAF-3 Antibody [NB100-56176]

Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Applications for TRAF-3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:4000

Immunohistochemistry-Paraffin

1:4000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF-3

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor super families and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis.

Long Name

TNF Receptor-Associated Factor 3

Alternate Names

CAP1, CD40bp, CRAF1, LAP1, TRAF3

Entrez Gene IDs

7187 (Human); 22031 (Mouse); 362788 (Rat)

Gene Symbol

TRAF3

Additional TRAF-3 Products

Product Documents for TRAF-3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAF-3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAF-3 Antibody - BSA Free

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Protocols

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