TRAF-5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87121

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAF-5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NAKVILGRYQQDHLQQCLFQPVQCSNEKCREPVLRKDLKEHLSASCQFRKEKCLYCKKDVVVINLQNHEENLCPEYPVFCPNNCAKIILKTEVDEHLAVCPEAEQDCPFKHYGCAVTDKR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAF-5 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRAF-5 Antibody [NBP1-87121]

Immunocytochemistry/ Immunofluorescence: TRAF-5 Antibody [NBP1-87121]

Immunocytochemistry/Immunofluorescence: TRAF-5 Antibody [NBP1-87121] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121]

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121]

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121]

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121]

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121] - Staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in red pulp.
Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121]

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121]

Immunohistochemistry-Paraffin: TRAF-5 Antibody [NBP1-87121] - Staining in human spleen and liver tissues using anti-TRAF5 antibody. Corresponding TRAF5 RNA-seq data are presented for the same tissues.

Applications for TRAF-5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRAF-5

The TRAF (TNF receptor-associated factor) family is a group of adapter proteins (TRAFs 1-6) that link a wide variety of cell surface receptors to diverse signaling cascades leading to the activation of NF-kB and mitogen-activated protein kinases (reviewed in Chung et al, 2002). TRAFs are major signal transducers for both the TNF and IL- 1/TLR receptor superfamilies and collectively play important functions in both adaptive and innate immunity. The carboxy-terminal region of TRAFs is required for self-association and interaction with receptor cytoplasmic domains following ligand-induced oligomerization. TRAFs interact with a variety of proteins that regulate receptor-induced cell death or survival, and TRAF-mediated signaling can promote cell survival or interfere with death receptor-induced apoptosis. IMG-5765 recognizes TRAF5. Mouse TRAF5 is a 558 amino acid protein.

Long Name

TNF Receptor-Associated Factor 5

Alternate Names

RNF84, TRAF5

Gene Symbol

TRAF5

Additional TRAF-5 Products

Product Documents for TRAF-5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAF-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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