TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74578

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2A5

Format

Azide and BSA Free
View all TRAIL R2/TNFRSF10B Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 203-411 of human TNFRSF10B(NP_671716) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

Western Blot: TRAILR2/TNFRSF10B Antibody (OTI2A5)Azide and BSA Free [NBP2-74578]

Western Blot: TRAILR2/TNFRSF10B Antibody (OTI2A5)Azide and BSA Free [NBP2-74578]

Western Blot: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF10B.
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Carcinoma of Human kidney tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Carcinoma of Human thyroid tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578]

Immunohistochemistry: TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free [NBP2-74578] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:200-500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TRAIL R2/TNFRSF10B

Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently (1-2). Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-kB.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2

Gene Symbol

TNFRSF10B

Additional TRAIL R2/TNFRSF10B Products

Product Documents for TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRAILR2/TNFRSF10B Antibody (OTI2A5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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