Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Flow (Cell Surface)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 12A380

Format

Azide and BSA Free
View all TRANCE/TNFSF11/RANK L Primary Antibodies »

Product Specifications

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE was used as immunogen.

Localization

Flow Cytometry (Surface): please see Yoneda et al. 2004 for details. Immunohistochemistry-Frozen: please see Yoneda et al. 2004 for details.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.
Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380)Azide and BSA Free [NBP2-27402]

Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380)Azide and BSA Free [NBP2-27402]

Western Blot: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - analysis of recombinant RANKL (partial) probed with RANKL antibody at 1 ug/ml.
Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Immunohistochemistry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis of FFPE human tonsil using TRANCE (12A380) antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining was observed. Staining was performed by Histowiz. Image using the standard format of this product.
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis in RAW cells using 2 ug of PE conjugated antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; purple represents antibody.
Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free [NBP2-27402]

Flow Cytometry: TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide Free [NBP2-27402] - Analysis using the Alexa Fluor (R) 647 conjugate. Staining of RANKL in untreated mouse splenocytes (green) and 72 hour ConA-stimulated mouse splenocytes (red) using this antibody. 1 ug of RANKL antibody was used for this assay.

Applications for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Frozen

1:10-1:2000

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 18705798)

Western Blot

1-5ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRANCE/TNFSF11/RANK L

TRANCE/OPGL/RANkL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANkL {receptor activator of NF-kB (RANk) ligand} has been implicated in interactions between T cells and dendritic cells (4-5). RANk ligand (RANkL/TRANCE) binds to RANk on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (4). TRANCE/RANkL is also important in T- and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (1,2). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Long Name

TNF-related Activation-induced Cytokine

Alternate Names

CD254, ODF, OPGL, RANK L, RANKL, TNFSF11

Entrez Gene IDs

21943 (Mouse)

Gene Symbol

TNFSF11

Additional TRANCE/TNFSF11/RANK L Products

Product Documents for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRANCE/TNFSF11/RANK L Antibody (12A380) - Azide and BSA Free

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Protocols

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