Transcription factor E3 Antibody (CL12822) - BSA Free

Novus Biologicals | Catalog # NBP3-18540

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL12822

Format

BSA Free
View all Transcription factor E3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P19532, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Transcription factor E3 Antibody (CL12822) - BSA Free

Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunocytochemistry/ Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunocytochemistry/Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of U2-OS cells using the Anti-TFE3 monoclonal antibody, showing specific staining in nucleoplasm in green. Microtubule probes are visualized in red (where available).
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human kidney cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540]

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.

Applications for Transcription factor E3 Antibody (CL12822) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 -1:2500

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transcription factor E3

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Alternate Names

BHLHE33, bHLHe33transcription factor E3, Class E basic helix-loop-helix protein 33, RCCP2, TFEA, transcription factor binding to IGHM enhancer 3, transcription factor E family, member A, Transcription factor for IgH enhancer

Gene Symbol

TFE3

Additional Transcription factor E3 Products

Product Documents for Transcription factor E3 Antibody (CL12822) - BSA Free

Certificate of Analysis

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Product Specific Notices for Transcription factor E3 Antibody (CL12822) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Transcription factor E3 Antibody (CL12822) - BSA Free

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Protocols

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FAQs

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