Transducin alpha Antibody (HL2126)

Novus Biologicals | Catalog # NBP3-48672

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2126
View all Transducin alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Transducin alpha

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Transducin alpha Antibody (HL2126)

Transducin alpha Antibody (HL2126)

Western Blot: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transducin alpha Antibody (HL2126)

Western Blot: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transducin alpha Antibody (HL2126)

Immunohistochemistry-Paraffin: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Transducin alpha antibody [HL2126] detects Transducin alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: Transducin alpha stained by Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Transducin alpha Antibody (HL2126)

Western Blot: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transducin alpha Antibody (HL2126)

Western Blot: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transducin alpha Antibody (HL2126)

Western Blot: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Transducin alpha Antibody (HL2126)

Immunohistochemistry-Paraffin: Transducin alpha Antibody (HL2126) [NBP3-48672] -

Transducin alpha antibody [HL2126] detects Transducin alpha protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: Transducin alpha stained by Transducin alpha antibody [HL2126] (NBP3-48672) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for Transducin alpha Antibody (HL2126)

Application
Recommended Usage

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Transducin alpha

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

Alternate Names

CSNBAD3, GBT1, GNATR, guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1, guanine nucleotide-binding protein G(t) subunit alpha-1, guanine nucleotide-binding protein G(T), alpha-1 subunit, rod-type transducin alpha subunit, Transducin alpha-1 chain, transducin, rod-specific

Gene Symbol

GNAT1

Additional Transducin alpha Products

Product Documents for Transducin alpha Antibody (HL2126)

Certificate of Analysis

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Product Specific Notices for Transducin alpha Antibody (HL2126)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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