Transducin alpha Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20696

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (98%), Canine (99%), Chicken (96%), Guinea Pig (99%), Human (90%), Rat (98%), Xenopus (92%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Transducin alpha Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transducin alpha. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transducin alpha Antibody - BSA Free

Transducin alpha Antibody - BSA Free

Western Blot: Transducin alpha Antibody [NBP2-20696] -

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [N1C3] (NBP2-20696) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Transducin alpha Antibody - BSA Free

Immunohistochemistry-Frozen: Transducin alpha Antibody [NBP2-20696] -

GNAT1 antibody [N1C3] detects GNAT1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GNAT1 protein stained by GNAT1 antibody [N1C3] (NBP2-20696) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250.
Blue: Fluoroshield with DAPI.

Applications for Transducin alpha Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Transducin alpha

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Alternate Names

CSNBAD3, GBT1, GNATR, guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1, guanine nucleotide-binding protein G(t) subunit alpha-1, guanine nucleotide-binding protein G(T), alpha-1 subunit, rod-type transducin alpha subunit, Transducin alpha-1 chain, transducin, rod-specific

Gene Symbol

GNAT1

UniProt

P11488 (Human)

Additional Transducin alpha Products

Product Documents for Transducin alpha Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Transducin alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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