Key Product Details

Accession Number

Q06AH7

Conjugate

HRP

Applications

Immunohistochemistry, Western Blot, ELISA, Dot Blot
View all Transferrin Proteins and Enzymes »

Product Specifications

Description

Native Human Transferrin

Source: Normal serum (Accession #:Q06AH7)

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Purity

Multi-step

Applications

Immunohistochemistry (1:200 - 1:2000)
Western Blot (1:2000 - 1:5000)
ELISA (1:4000 - 1:10000)
Dot Blot (1:100 - 1:2000)

Application Notes

This product has been tested in dot blot and is a suitable protein for use as a control reagent in both Western Blotting and ELISA experiments.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Protein / Peptide Type

Native Protein

Scientific Data Images for Transferrin Native Protein

Dot Blot: Transferrin Native Protein [NBP1-97069] - Dot Blot result of Human Transferrin Peroxidase conjugate. Dots are Human Transferrin HRP at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Block for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.
Transferrin Native Protein

Transferrin Native Protein

Dot Blot result of Transferrin Native Protein. Dots are Human Transferrin HRP at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 60 min at RT. Primary Antibody: none. Secondary Antibody: none. Imaged with BioRad ChemiDoc, Chemi filter.

Formulation, Preparation, and Storage

NBP1-97069
Preparation Method This product was prepared from normal serum by a multi-step process including selective precipitation and tandem chromatography followed by extensive dialysis against the buffer stated above. Human Transferrin Peroxidase conjugated was assayed by immunoelectrophoresis and resulted in a single precipitin arc against anti-Peroxidase, anti-Human Transferrin and anti-Human Serum.
Formulation Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% Gentamicin Sulfate
Concentration Lyoph
Reconstitution Reconstitute with 1.0 ml deionized water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Transferrin

Human transferrin is encoded by the TF gene and is an iron-binding blood plasma glycoprotein that controls the level of free iron in biological fluids. Human transferrin binds iron very tightly but reversibly. Human transferrin is the most important iron pool in mammals. Human transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of Human transferrin for Fe(III) is extremely high but decreases progressively with decreasing pH below neutrality. Human Transferrin also plays a role in the immune system, creating environments low in iron for which many pathogenic bacteria are unable to thrive.

Alternate Names

Beta-1 metal-binding globulin, Serotransferrin, Siderophilin, TF

Gene Symbol

TF

UniProt

Q06AH7 (Human)

Additional Transferrin Products

Product Documents for Transferrin Native Protein

Certificate of Analysis

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Product Specific Notices for Transferrin Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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