Transglutaminase 2/TGM2 Antibody

Novus Biologicals | Catalog # NBP2-20698

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Transglutaminase 2/TGM2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Guinea pig (85%), Bovine (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Transglutaminase 2/TGM2 Antibody

Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-20698]

Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-20698]

Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Mouse duodenum. Transglutaminase 2 antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody [NBP2-20698] - Mahlarvu xenograft, using Transglutaminase 2 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Transglutaminase 2/TGM2 Antibody

Western Blot: Transglutaminase 2/TGM2 Antibody [NBP2-20698] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Transglutaminase 2 antibody [N1N3] (NBP2-20698) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Transglutaminase 2/TGM2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Transglutaminase 2/TGM2

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

TGC, TGM2, Transglutaminase 2, tTG

Gene Symbol

TGM2

UniProt

Additional Transglutaminase 2/TGM2 Products

Product Documents for Transglutaminase 2/TGM2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Transglutaminase 2/TGM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Transglutaminase 2/TGM2 Antibody

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Protocols

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