TRAP220/MED1 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-2574

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Mouse (100%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Chromatin Immunoprecipitation (ChIP), IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAP220 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1525 and the C-terminus (residue 1581) of human Cofactor Required for Sp1 Transcriptional Activation 1using the numbering given in entry NP_004765.2 (GeneID 5469).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRAP220/MED1 Antibody - BSA Free

Western Blot: TRAP220/MED1 Antibody [NB100-2574]

Western Blot: TRAP220/MED1 Antibody [NB100-2574]

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (50 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574]

Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574]

Immunohistochemistry-Paraffin: TRAP220/MED1 Antibody [NB100-2574] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MED1 antibody used at 1:5000 (0.2ug/ml). Secondary: HRP-conjugated goat anti-rabbit IgG
Western Blot: TRAP220/MED1 Antibody [NB100-2574]

Western Blot: TRAP220/MED1 Antibody [NB100-2574]

Western Blot: TRAP220/MED1 Antibody [NB100-2574] - Whole cell lysate (10 ug) from RKO, HeLa, HEK293T, K-562, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MED1 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposuretime of 3 seconds.
Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574]

Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574]

Immunoprecipitation: TRAP220/MED1 Antibody [NB100-2574] - Detection of human MED1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MED1 antibody NB100-2574(lot NB100-2574-9) used for IP at 6 ug per reaction. For blotting immunoprecipitated MED1, NB100-2574 was used at 1:1000. Detection: Chemiluminescence with an exposure time time of 30 seconds.

Applications for TRAP220/MED1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:2000 -1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. Knockdown validation (PMID: 31695025).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TRAP220

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53-dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize. [provided by RefSeq]

Long Name

Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa

Alternate Names

DRIP230, MBD4, MED1, PPARBP, PPARGBP, TRIP2

Entrez Gene IDs

5469 (Human); 19014 (Mouse)

Gene Symbol

MED1

UniProt

NP_004765.2 (Human)

Additional TRAP220 Products

Product Documents for TRAP220/MED1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAP220/MED1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAP220/MED1 Antibody - BSA Free

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Protocols

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