TRAPPC9 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-55735

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Canine, Chicken, Primate

Predicted:

Xenopus (94%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRAPPC9 Primary Antibodies »

Product Specifications

Immunogen

Amino acids 282-299 (KKDFVGLDTDSRHYKKRC) of human NIBP was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: 100% homologous in human (isoform CRA_A and CRA_b), chimpanzee, monkey, mouse, opossum, rat and dog; xenopus (94%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for TRAPPC9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (Zahoor et al (2010))

Immunoprecipitation

1:10-1:500. Use reported in scientific literature (Zahoor et al (2010))

Western Blot

1:100-1:2000. Use reported in scientific literature (Zahoor et al (2010))
Application Notes
Confocal immunofluorescence: Cells were fixed with 4% paraformaldehyde in PBS for 10 min and then permeabilized with 0.5 % Triton X-100 for 20 min at room temperature (RT). The cells were blocked with PBS containing 10 % BSA and probed with NIBP pAB for 1 hour at RT. After washing with PBS, cells were incubated with Alexa conjugated goat anti-mouse antibodies. See Zahoor et al for details (2010).

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole Rabbit antisera with 50% Glycerol

Format

BSA Free

Preservative

0.025% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRAPPC9

NIBP is a 1246 amino acid intracellular regulatory protein. Reports suggest that NIBP physically interacts with NIK, IKK (beta), but not IKK (alpha) or IKK (gamma) and thus acts as a novel enhancer of cytokine-induced NF-(kappa) B signaling pathway. Due to its neuronal expression NIBP is considered as a potential target for modulating the NF-(kappa) B signaling cascade in neuronal pathologies dependent upon abnormal activation of this pathway. NIBP is mainly expressed in brain, muscle, heart, and kidney.

Alternate Names

IKBKBBP, IKK2 binding protein, KIAA1882IBP, MRT13MGC4769, NIBPMGC4737, NIK- and IKBKB-binding protein, NIK and IKK-beta binding protein, T1, trafficking protein particle complex 9, trafficking protein particle complex subunit 9, TRAPP 120 kDa subunit, TRS120, Tularik gene 1 protein

Entrez Gene IDs

83696 (Human)

Gene Symbol

TRAPPC9

UniProt

Q96Q05 (Human)

Additional TRAPPC9 Products

Product Documents for TRAPPC9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRAPPC9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRAPPC9 Antibody - BSA Free

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Protocols

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