TREM2 Antibody

Immunocytochemistry/Immunofluorescence: Goat Polyclonal TREM2 Antibody [NBP1-44067] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal TREM2 Antibody [NBP1-44067] - Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunocytochemistry/Immunofluorescence: Goat Polyclonal TREM2 Antibody [NBP1-44067] -

Immunocytochemistry/Immunofluorescence: Goat Polyclonal TREM2 Antibody [NBP1-44067] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western Blot: TREM2 Antibody [NBP1-44067] -

Repression of microglial primary cilia in 5xFAD mice (A) Representative images of AC3 (green)-positive microglia (MG) stained for Iba1 (red) in the LS and the cortex of WT and 5xFAD mice (6-month-old). In 5xFAD mice, A beta plaques were stained using 6E10 antibodies (white). (B) Plots depict the percentage of AC3-positive microglia and the length of AC3-stained primary cilia, respectively. Images were taken from the LS (1024 ㎛2) and the cortex (1024 ㎛2) of WT and 5xFAD mice (5-month-old, n = 4, each group, male) (C) Western blot analysis shows the differential expression of the homeostatic microglial markers in BV2 cells, which were harvested 24 h after transfection with siIft88. Plots represent protein expression normalized by alpha -tubulin ( alpha Tub, n = 3). (D) Western blot analysis shows the differential expression of the DAM markers in BV2 cells transfected with siIft88 for 24 h. Plots represent protein expression normalized by alpha Tub (n = 3). (B–D) Data represent means +/- SEM, and p-values were calculated by an unpaired two-tailed t-test. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns (non-significant). Scale bars = 10 um. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37942288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.