Treponema pallidum Antibody - BSA Free

Novus Biologicals | Catalog # NB110-17242

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Highly Purified T. pallidum

Epitope

>95% pure. Sodium sulfate precipitation and DEAE chromatography.

Reactivity Notes

Recognizes all antigens. Non-reactive in RPR assay. Has not been absorbed and may react with related microorganisms.

Localization

Whole virus

Specificity

Recognizes all antigens. Non-reactive in RPR assay. Has not been absorbed and may react with related microorganisms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Treponema pallidum Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
Other applications have not been tested but use in such assays should not necessarily be excluded.

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

10mM PBS (pH 7.2)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

4.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Treponema pallidum

Alternate Names

T. pallidum

Additional Treponema pallidum Products

Product Documents for Treponema pallidum Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Treponema pallidum Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Treponema pallidum Antibody - BSA Free

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  • Q: I would like to use this antibody for IHC-P, and would like to know the recommended Antigen Retrieval Method.

    A: This antibody is for detecting the specimens that have been infected with the spirochaete pathogen. Unlike the cellular proteins (targeting by many antibodies) whose locations in vivo are strictly defined by their own biological, physiological, pathological, and others properties, Treponema pallidum can actually move around in the hosts after infection (T. pallidum is a motile spirochaete that, entering the host via breaches in squamous or columnar epithelium.  The helical structure of T. pallidum allows it to move in a corkscrew motion through a viscous medium such as mucus. It gains access to the host's blood and lymph systems through tissue and mucous membranes. Therefore, there is no consensual protocol regarding the antigen retrieval method for detecting anti-Treponema pallidum in the IHC-P tissues. However, the most publications/websites posted online suggest using the heat-induced methods, and at least two have recommended the citrate buffer/HIER Citrate Buffer (pH 6.5).

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