TRF-1 Antibody (57-6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80993

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 57-6

Format

Azide and BSA Free
View all TRF-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human TRF1 [UniProt# P54274]

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRF-1 Antibody (57-6) - Azide and BSA Free

Western Blot: TRF-1 Antibody (57-6)Azide and BSA Free [NBP2-80993]

Western Blot: TRF-1 Antibody (57-6)Azide and BSA Free [NBP2-80993]

Western Blot: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993] - Detection of TRF1. 50 ug of total lysate each lane. Image from the standard format of this antibody.
Flow Cytometry: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993]

Flow Cytometry: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993]

Flow Cytometry: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using TRF1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.
Western Blot: TRF-1 Antibody (57-6)Azide and BSA Free [NBP2-80993]

Western Blot: TRF-1 Antibody (57-6)Azide and BSA Free [NBP2-80993]

Western Blot: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993] - Detection of TRF1 in HeLa whole cell extracts. Image from the standard format of this antibody.
Flow Cytometry: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993]

Flow Cytometry: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993]

Flow Cytometry: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993] - An intracellular stain was performed on HeLa with NB110-68281 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image from the standard format of this antibody.
Simple Western: TRF-1 Antibody (57-6)Azide and BSA Free [NBP2-80993]

Simple Western: TRF-1 Antibody (57-6)Azide and BSA Free [NBP2-80993]

Simple Western: TRF-1 Antibody (57-6) - Azide and BSA Free [NBP2-80993] - Image shows a specific band for TRF1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Image from the standard format of this antibody.

Applications for TRF-1 Antibody (57-6) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1 ug per million cells

Simple Western

1:200

Western Blot

0.5 ug/ml
Application Notes
This TRF1 antibody is useful for ELISA and Western blot, where a band can be seen at approx. 60 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:200. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine, 0.15M NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRF-1

TRF1 (telomeric repeat-binding factor 1) is a protein that binds telomeric double-stranded TTAGGG repeat and is implicated in telomere replication, telomere protection, telomere length maintenance, resolution of sister telomeres and mitotic spindle regulation. TRF1 interacts directly with TIN2 in shelterin complex (TRF1, TRF2, TIN2, POT1, TPP1 and hRap1; a complex that coats telomeres for their protection and regulation of telomere length) and it then binds to TRF2/TPP1. While POT1 binds to TPP1, TRF2 interacts and forms a complex with hRap1. Upon ionizing DNA damage, TRF1 gets phosphorylated (ser-219) in ATM-dependent manner. ADP-ribosylation by TNKS1 or TNKS2 diminishes TRF1's telomeric DNA binding ability, whereas, RLIM/RNF12 as well as SCF (SKP1-CUL1-F-box protein) can ubiquinate TRF1 for its degradation. TRF1 is ubiquitously expressed and during the cell cycle, its levels are low in G1/S phases which may increase during G2/M phases. Overexpression of TRF1 promotes telomere shortening whereas loss of TRF1 accumulate telomere fusions and induce telomerase-dependent telomere lengthening, implying that TRF1 negatively regulates telomerase-dependent telomere extension, perhaps by restricting the access of telomerase to the ends of telomeres. TRF1 knockout leads to embroynic lethality and deletion of TRF1 promotes the formation of fragile telomeres.

Long Name

Telomeric Repeat Binding Factor

Alternate Names

PIN-2, TERF1, TRF1

Gene Symbol

TERF1

Additional TRF-1 Products

Product Documents for TRF-1 Antibody (57-6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TRF-1 Antibody (57-6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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