TRF-1 Antibody (TRF-78) - BSA Free

Novus Biologicals | Catalog # NB100-1701

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Microarray

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TRF-78

Format

BSA Free
View all TRF-1 Primary Antibodies »

Product Specifications

Immunogen

Human TRF1 protein produced in baculovirus.

Localization

Nuclear

Marker

Telomere Marker

Specificity

TRF1 (TRF-78)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRF-1 Antibody (TRF-78) - BSA Free

Western Blot: TRF-1 Antibody (TRF-78)BSA Free [NB100-1701]

Western Blot: TRF-1 Antibody (TRF-78)BSA Free [NB100-1701]

Western Blot: TRF-1 Antibody (TRF-78) [NB100-1701] - Cell line lysates were separated on SDS-PAGE and probed with 4 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. Lane 1: HeLa Nuclear, Lane 2: HeLa, Lane 3: HEK-293T, Lane 4: U2OS, Lane 5: HepG2, Lane 6: P19, Lane 7: PC12.
Immunocytochemistry/ Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701]

Immunocytochemistry/ Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701]

Immunocytochemistry/Immunofluorescence: TRF-1 Antibody (TRF-78) - BSA Free [NB100-1701] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-TRF1 Clone: TRF-78. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Applications for TRF-1 Antibody (TRF-78) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

2-4 ug/ml
Application Notes
In Western Blot a 70 kDa band is seen.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRF-1

TRF1 binds the double-stranded telomeric repeat sequence, TAGGG. TRF1 and TRF2 are key components of vertebrate telomeres and bind to double-stranded telomeric DNA as homodimers. Dimerization involves the TRF homology (TRFH) domain, which also mediates interactions with other telomeric proteins. Fairall et al. (2001) determined the crystal structures of the dimerization domains from human TRF1 and TRF2 at 2.9- and 2.2-angstrom resolution, respectively. Despite a modest sequence identity, the 2 TRFH domains have the same entirely alpha-helical architecture, resembling a twisted horseshoe. The dimerization interfaces feature unique interactions that prevent heterodimerization. Mutational analysis of TRF1 corroborated the structural data and underscored the importance of the TRFH domain in dimerization, DNA binding, and telomere localization.

Long Name

Telomeric Repeat Binding Factor

Alternate Names

PIN-2, TERF1, TRF1

Entrez Gene IDs

7013 (Human)

Gene Symbol

TERF1

UniProt

P54274

Additional TRF-1 Products

Product Documents for TRF-1 Antibody (TRF-78) - BSA Free

Certificate of Analysis

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Product Specific Notices for TRF-1 Antibody (TRF-78) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRF-1 Antibody (TRF-78) - BSA Free

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Protocols

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