TRF-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86911

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRF-2 Primary Antibodies »

Product Specifications

Immunogen

This TRF-2 Antibody antibody was developed against Recombinant Protein corresponding to amino acids: STGKEDKQPAPGPVEKPPREPARQLRNPPTTIGMMTLKAAFKTLSGAQDSEAAFAKLDQKDLVLPTQALPASPALKNKRPRKDENESSAPADGEGGSELQPKNKRMTISRLVLEEDSQSTEPS

Marker

Telomeres marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TRF-2 Antibody - BSA Free (NBP1-86911) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TRF-2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NBP1-86911]

Immunocytochemistry/ Immunofluorescence: TRF-2 Antibody [NBP1-86911]

Immunocytochemistry/Immunofluorescence: TRF-2 Antibody [NBP1-86911] - Staining of human cell line U-2 OS shows localization to nuclear bodies. Antibody staining is shown in green.
Western Blot: TRF-2 Antibody [NBP1-86911]

Western Blot: TRF-2 Antibody [NBP1-86911]

Western Blot: TRF-2 Antibody [NBP1-86911] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
TRF-2 Antibody - BSA Free Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Staining of human cerebral cortex, gastrointestinal).
TRF-2 Antibody - BSA Free Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Staining of human tonsil shows strong nuclear positivity in germinal center cells.
TRF-2 Antibody - BSA Free Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
TRF-2 Antibody - BSA Free Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
TRF-2 Antibody - BSA Free Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Immunohistochemistry-Paraffin: TRF-2 Antibody - BSA Free [NBP1-86911]

Staining of human colon shows strong nuclear positivity in glandular cells.

Applications for TRF-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRF-2

Originally discovered as part of shelterin complex, telomeric repeat-binding factor 2 (TRF2, also called TERF2 or TRB2) is a ubiquitously expressed nuclear protein (55-60 kDa) involved in telomere homeostasis. TRF2 contains an N-terminal GAR domain, a central TRFH dimerization domain, and a C-terminal SAND/MYB-type DNA binding domain. Trf2 RNA has 10 exons and alternative splicing in rodents produces a truncated form, TRF2-S, which lacks the DNA binding domain and nuclear localization signal (NLS) (1).

Both TRF2 and TRF1 bind to telomeric double stranded 5'-TTAGGG-3' DNA repeats, then recruit RAP1, TIN2, TPP1, and POT1 for the assembly of the shelterin complex. The telomeric association of TRF2 is greatly increased in the S phase of the cell cycle (2). Loss of TRF2 leads to telomere shortening, the DNA damage response, chromosomal instability, and replicative senescence. Interestingly, the contribution of TRF2 to telomere shortening via a telomerase-independent mechanism has also been reported (3). In conjunction with the exonuclease, Apollo, TRF2 protects telomeres during replication and negatively regulates the accumulation of DNA topoisomerase (TOP1, TOP2A and TOP2B).

TRF2 has been implicated in cancer, shown to be a major oncogene in telomerase-deficient mice. A link to Werner syndrome, a premature aging disease caused by the loss of WRN, has been reported based on TRF2 recruitment of WRN for processing of telomeric DNA (4). TRF2 expression is increased during human embryonic stem cell differentiation and has been shown to interact with Repressor Element-1 Silencing Transcription Factor (REST), protecting it from proteasomal degradation (5).

References

1. Grammatikakis, I., Zhang, P., Mattson, M. P., & Gorospe, M. (2016). The long and the short of TRF2 in neurogenesis. Cell cycle (Georgetown, Tex.), 15(22), 3026-3032. PMID: 27565210

2. Li, F., Kim, H., Ji, Z., Zhang, T., Chen, B., Ge, Y., Hu, Y., Feng, X., Han, X., Xu, H., Zhang, Y., Yu, H., Liu, D., Ma, W., & Songyang, Z. (2018). The BUB3-BUB1 Complex Promotes Telomere DNA Replication. Molecular cell, 70(3), 395-407. PMID: 29727616

3. Ancelin, K., Brunori, M., Bauwens, S., Koering, C. E., Brun, C., Ricoul, M., Pommier, J. P., Sabatier, L., & Gilson, E. (2002). Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2. Molecular and cellular biology, 22(10), 3474-3487. PMID: 11971978

4. Machwe A, Xiao L, & Orren DK. (2004) TRF2 recruits the Werner syndrome (WRN) exonuclease for processing of telomeric DNA. Oncogene. 23(1):149-56. PMID: 14712220.

5. Diotti, R., & Loayza, D. (2011). Shelterin complex and associated factors at human telomeres. Nucleus (Austin, Tex.), 2(2), 119-135. PMID: 21738835

Long Name

Telomeric Repeat Binding Factor 2

Alternate Names

TERF2, TRBF2, TRF2

Gene Symbol

TERF2

Additional TRF-2 Products

Product Documents for TRF-2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRF-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

p53 Pathway

Citations for TRF-2 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TRF-2 Antibody - BSA Free

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    • A: Yes, we offer a 2 TRF-2 antibodies that have been tested in Simple Western: NB100-56506 and NB110-57130.

    • A: we carry 2 TERF2/TRBF2 antibodies in lyophilized form: AF5635, MAB5635.

    • A: The TMW of TRF-2 antibodies is approximately 55 - 56 kDa.
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