Triadin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-49277

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Triadin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: TEITAEGNASTTTTVIDSKNGSVPKSPGKVLKRTVTEDIVTTF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Triadin Antibody - BSA Free

Triadin Antibody

Immunohistochemistry: Triadin Antibody [NBP2-49277] -

Immunohistochemistry: Triadin Antibody [NBP2-49277] - Analysis in human skeletal muscle and skin tissues using NBP2-49277 antibody. Corresponding TRDN RNA-seq data are presented for the same tissues.
Triadin Antibody

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] -

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Triadin Antibody

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] -

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] - Staining of human heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.
Triadin Antibody

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] -

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.
Triadin Antibody

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] -

Immunohistochemistry-Paraffin: Triadin Antibody [NBP2-49277] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

Applications for Triadin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Triadin

The junction between the transverse tubules (T-tubules) and the sarcoplasmic reticulum (SR) of skeletal muscle is called the triad. At the triad, dihydropyridine receptors (DHPR®s) of the T-tubule serve as voltage sensors in excitation-contraction coupling, while ryanodine receptors (RyR®s), the calcium release channels, exist in the membrane of the terminal cisternae of the SR. It is thought that during slow phase depolarization of the T-tubule, a third protein, Triadin (MW 95 kDa) transmits electrochemical signals to the SR through direct interaction with both DHPR®s and RyR®s. Though its exact role in this signaling process is unclear, triadin has been shown to co-localize with both DHPR and RYR at the junctional face of the terminal cisternae.

Alternate Names

dJ166D18.1 (triadin), TDN, triadin, TRISK

Gene Symbol

TRDN

Additional Triadin Products

Product Documents for Triadin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Triadin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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