TRIB1 Antibody - BSA Free

Western Blot: TRIB1 Antibody [NBP1-55386]

Western Blot: TRIB1 Antibody [NBP1-55386] - Sample Tissue: Human Stomach Tumor Antibody Dilution: 1.0 ug/ml

Immunohistochemistry: TRIB1 Antibody - BSA Free [NBP1-55386] -

Trib1 expression was downregulated during the early phase of liver regeneration.A–C C57B6/L mice were subjected to partial hepatectomy (2/3 PHx) and sacrificed at indicated time points. Trib1 expression was examined by qPCR, western blotting, and immunohistochemical staining. N = 5 mice for each group. D–F C57B6/L mice were injected with acetaminophen (APAP, 300 mg/kg) and sacrificed at indicated time points. Trib1 expression was examined by qPCR, western blotting, and immunohistochemical staining. N = 5 mice for each group. G, H Primary murine hepatocytes were treated with HGF and harvested at indicated time points. Trib1 expression was examined by qPCR and western blotting. N = 3 biological replicates. Data are expressed as mean +/- S.D. *p < 0.05, one-way ANOVA with post-hoc Scheff´e. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37355685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: TRIB1 Antibody - BSA Free [NBP1-55386] -

Trib1 expression was downregulated during the early phase of liver regeneration.A–C C57B6/L mice were subjected to partial hepatectomy (2/3 PHx) and sacrificed at indicated time points. Trib1 expression was examined by qPCR, western blotting, and immunohistochemical staining. N = 5 mice for each group. D–F C57B6/L mice were injected with acetaminophen (APAP, 300 mg/kg) and sacrificed at indicated time points. Trib1 expression was examined by qPCR, western blotting, and immunohistochemical staining. N = 5 mice for each group. G, H Primary murine hepatocytes were treated with HGF and harvested at indicated time points. Trib1 expression was examined by qPCR and western blotting. N = 3 biological replicates. Data are expressed as mean +/- S.D. *p < 0.05, one-way ANOVA with post-hoc Scheff´e. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37355685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: TRIB1 Antibody - BSA Free [NBP1-55386] -

Correlation between TRIB1 expression, NRF2 nuclear localization, and hepatocyte proliferation in human ALF specimens.A Human liver biopsy specimens were stained with anti-TRIB1, anti-NRF2, and anti-PCNA. B Linear regression was performed with Graphpad. N = 9. C A schematic model. In quiescent hepatocytes, LXR alpha binds to the Trib1 promoter and activates Trib1 transcription. Trib1 interacts with Nrf2 to sequester Nrf2 in the cytoplasm thus turning off the transcription of antioxidant genes. In proliferating hepatocytes during live regeneration, deactivation of LXR alpha shuts down Trib1 transcription, which unleashes Nrf2 from sequestration. Nrf2 translocates to the nucleus and activates the transcription of antioxidant genes to modulate intracellular redox status fueling cell proliferation. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37355685), licensed under a CC-BY license. Not internally tested by Novus Biologicals.