TRIM2 Antibody

Novus Biologicals | Catalog # NB100-1218

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIM2 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence DSGNHCFKVYRYLQ, from the C Terminus of the protein sequence according to NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1.

Epitope

C-DSGNHCFKVYRYLQ

Reactivity Notes

Use in Mouse reported in secitific publication PMID: 32698497

Specificity

This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM2 Antibody

Western Blot: TRIM2 Antibody [NB100-1218]

Western Blot: TRIM2 Antibody [NB100-1218]

Western Blot: TRIM2 Antibody [NB100-1218] - Using 0.5ug/ml of this antibody, the staining of Rat Brain (A) + Peptide (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218]

Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218]

Immunohistochemistry-Paraffin: TRIM2 Antibody [NB100-1218] - Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: TRIM2 Antibody [NB100-1218]

Flow Cytometry: TRIM2 Antibody [NB100-1218]

Flow Cytometry: TRIM2 Antibody [NB100-1218] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for TRIM2 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunohistochemistry

6 - 12 ug/ml

Immunohistochemistry-Paraffin

6 - 12 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.5 - 1 ug/ml
Application Notes
IP successful usage on mouse protein is reported in scientific literature (PMID: 21478148). WB: ~ 75-85kDa bands observed in rat brain lysates (calculated MW of 81.5 kDa band according to human NP_001123539.1, 84.4 according to human NP_056086.2 and 83.3 according to rat NP_001102022.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human brain (cortex) shows cytoplasm stainning in neuronal cell bodies. Use in ICC/IF reported in secitific publication PMID: 32698497

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM2

This protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Alternate Names

KIAA0517tripartite motif protein 2, RING finger protein 86, RNF86tripartite motif protein TRIM2, tripartite motif containing 2, tripartite motif-containing 2, tripartite motif-containing protein 2

Entrez Gene IDs

23321 (Human); 80890 (Mouse)

Gene Symbol

TRIM2

UniProt

NP_056086.2 (Human), NP_001123539.1 (Human), NP_001337983.1 (Human), NP_001337984.1 (Human), NP_001337985.1 (Human), NP_001337986.1 (Human)

Additional TRIM2 Products

Product Documents for TRIM2 Antibody

Certificate of Analysis

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Product Specific Notices for TRIM2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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