TRIM27 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20711

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRIM27 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human TRIM27. The exact sequence is proprietary.

Localization

Nucleus (By similarity); Cytoplasm (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TRIM27 Antibody - BSA Free

Western Blot: TRIM27 Antibody [NBP2-20711]

Western Blot: TRIM27 Antibody [NBP2-20711]

Western Blot: TRIM27 Antibody [NBP2-20711] - TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody diluted at a dilution of 1:500.
Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody [NBP2-20711]

Immunocytochemistry/ Immunofluorescence: TRIM27 Antibody [NBP2-20711]

Immunocytochemistry/Immunofluorescence: TRIM27 Antibody [NBP2-20711] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TRIM27 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Western Blot: TRIM27 Antibody [NBP2-20711]

Western Blot: TRIM27 Antibody [NBP2-20711]

Western Blot: TRIM27 Antibody [NBP2-20711] - TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody diluted by 1:500.
Western Blot: TRIM27 Antibody [NBP2-20711]

Western Blot: TRIM27 Antibody [NBP2-20711]

Western Blot: TRIM27 Antibody [NBP2-20711] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody diluted at 1:500.
Immunoprecipitation: TRIM27 Antibody [NBP2-20711]

Immunoprecipitation: TRIM27 Antibody [NBP2-20711]

Immunoprecipitation: TRIM27 Antibody [NBP2-20711] - Immunoprecipitation of TRIM27 protein from Jurkat whole cell extracts using 5 ug of TRIM27 antibody. Western blot analysis was performed using TRIM27 antibody.

Applications for TRIM27 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM27

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq]

Alternate Names

Ret finger proteinRING finger protein 76, RFPtripartite motif protein TRIM27, RNF76RFP transforming protein, tripartite motif containing 27, tripartite motif-containing 27, Tripartite motif-containing protein 27, zinc finger protein RFP

Gene Symbol

TRIM27

UniProt

P14373 (Human)

Additional TRIM27 Products

Product Documents for TRIM27 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM27 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM27 Antibody - BSA Free

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Protocols

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FAQs

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