TRIM31 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93541

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRIM31 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 186-425 of human TRIM31 (NP_008959.3). ELLHQVLEEEKNFLLSRIYWLGHEGTEAGKHYVASTEPQLNDLKKLVDSLKTKQNMPPRQLLEDIKVVLCRSEEFQFLNPTPVPLELEKKLSEAKSRHDSITGSLKKFKDQLQADRKKDENRFFKSMNKNDMKSWGLLQKNNHKMNKTSEPGSSSAGGRTTSGPPNHHSSAPSHSLFRASSAGKVTFPVCLLASYDEISGQGASSQDTKTFDVALSEELHAALSEWLTAIRAWFCEVPSS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM31 Antibody - BSA Free

Western Blot: TRIM31 AntibodyBSA Free [NBP2-93541]

Western Blot: TRIM31 AntibodyBSA Free [NBP2-93541]

Western Blot: TRIM31 Antibody [NBP2-93541] - Analysis of extracts of various cell lines, using TRIM31 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.
TRIM31 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRIM31 Antibody - BSA Free [NBP2-93541] -

Immunocytochemistry/ Immunofluorescence: TRIM31 Antibody - BSA Free [NBP2-93541] - Immunofluorescence analysis of NIH-3T3 cells using TRIM31 Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for TRIM31 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM31

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to both the cytoplasm and the nucleus. Its function has not been identified.

Alternate Names

C6orf13E3 ubiquitin-protein ligase TRIM31, EC 6.3.2.-, HCG1, HCGItripartite motif-containing 31 gamma, RNFring finger protein, tripartite motif containing 31, tripartite motif-containing 31, Tripartite motif-containing protein 31

Gene Symbol

TRIM31

Additional TRIM31 Products

Product Documents for TRIM31 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRIM31 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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