TRIM5 Antibody

Novus Biologicals | Catalog # NB100-1226

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRIM5 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence ASGILVNVKEEVTC corresponding to N-Terminus according to NP_149023.1, NP_149083.1, NP_149084.1.

Specificity

This antibody is expected to recognise all three human isoforms of this protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRIM5 Antibody

Western Blot: TRIM5 Antibody [NB100-1226]

Western Blot: TRIM5 Antibody [NB100-1226]

Western Blot: TRIM5 Antibody [NB100-1226] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226]

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226]

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.
Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226]

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226]

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - Staining of paraffin embedded Human Colon. Antibody at 3 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for TRIM5 Antibody

Application
Recommended Usage

Immunohistochemistry

3 ug/mL

Immunohistochemistry-Paraffin

3 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

1 - 3 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRIM5

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined.

Long Name

Tripartite Motif-containing 5

Alternate Names

RNF88

Entrez Gene IDs

85363 (Human)

Gene Symbol

TRIM5

UniProt

NP_149023 (Human)

Additional TRIM5 Products

Product Documents for TRIM5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRIM5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Infectious Diseases

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Protocols

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FAQs

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