TRM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82197

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TRM1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRM1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRM1 Antibody [NBP1-82197]

Immunocytochemistry/ Immunofluorescence: TRM1 Antibody [NBP1-82197]

Immunocytochemistry/Immunofluorescence: TRMT1 Antibody [NBP1-82197] - Staining of human cell line U-2 OS shows positivity in plasma membrane and cytoplasm.
Immunohistochemistry-Paraffin: TRM1 Antibody [NBP1-82197]

Immunohistochemistry-Paraffin: TRM1 Antibody [NBP1-82197]

Immunohistochemistry-Paraffin: TRMT1 Antibody [NBP1-82197] - Staining of human tonsil shows strong cytoplasmic positivity in germinal center cells.

Applications for TRM1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRM1

Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

Alternate Names

EC 2.1.1.32, FLJ20244, N(2)-N(2)-dimethylguanosine tRNA methyltransferase, TRM1, TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae), tRNA 2,2-dimethylguanosine-26 methyltransferase, tRNA(guanine-26, N(2)-N(2)) methyltransferase, tRNA(m(2,2)G26)dimethyltransferase

Gene Symbol

TRMT1

UniProt

Q9NXH9 (Human)

Additional TRM1 Products

Product Documents for TRM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TRM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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