Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

Novus Biologicals | Catalog # NBP2-37536

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2F12G2

Format

BSA Free
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Product Specifications

Immunogen

Purified recombinant fragment of human Troponin I type 2 (fast skeletal) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2)BSA Free [NBP2-37536]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2)BSA Free [NBP2-37536]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against HEK293 (1) and TNNI2 (AA: 1-182)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free [NBP2-37536]

Immunocytochemistry/ Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free [NBP2-37536]

Immunocytochemistry/Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Immunofluorescence analysis of Hela cells using TNNI2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free [NBP2-37536]

Flow Cytometry: Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free [NBP2-37536]

Flow Cytometry: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Flow cytometric analysis of NIH/3T3 cells using TNNI2 mouse mAb (green) and negative control (red).
Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2)BSA Free [NBP2-37536]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2)BSA Free [NBP2-37536]

Western Blot: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Western blot analysis using TNNI2 mAb against human TNNI2 (AA: 1-182) recombinant protein. (Expected MW is 21 kDa)
ELISA: Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free [NBP2-37536]

ELISA: Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free [NBP2-37536]

ELISA: Troponin I type 2 (fast skeletal) Antibody (2F12G2) [NBP2-37536] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Troponin I type 2 (fast skeletal)

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Alternate Names

skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform

Gene Symbol

TNNI2

Additional Troponin I type 2 (fast skeletal) Products

Product Documents for Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Troponin I type 2 (fast skeletal) Antibody (2F12G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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