Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoassay, Sandwich ELISA, Immunoprecipitation, Immunoaffinity Purification

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 560cc

Format

BSA Free
View all Troponin I/TNNI3 Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human cTn complex.

Epitope

a.a.r. 83-93

Specificity

No cross-reaction with skeletal muscle TnI

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Troponin I Type 3 (cardiac) Antibody (560cc) - BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoaffinity Purification

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended pair for sandwich immunoassay (capture-detection): NB110-2546 - NB100-73087, or NB100-73087 - NB100-73086.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I/TNNI3

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human)

Gene Symbol

TNNI3

UniProt

P19429

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (560cc) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (560cc) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Troponin I Type 3 (cardiac) Antibody (560cc) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Troponin I Type 3 (cardiac) Antibody (560cc) - BSA Free

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  • Q: Would you please help suggest antibody pairs for detecting Human Creatine Kinase MB,.Myoglobin,.C-reactive protein, and Troponin I, as well as their positive controls? So far, I can only find NB120-8345 and NB600-1291 for Myoglobin. There seems no Creatine Kinase MB antibody available from Novus. I also found two pairs suggested for Troponin I: NB110-2546(Coating)- NB100-73082(Conjugate); NB100-73087(Coating) - NB110-2546(Conjugate). Would you please help confirm? How about CRP? May we also ask if NB810-73233 can be used as the antigen in ELISA of CRP?

    A:

    For Myoglobin The recommended antibodies are NB120-8345 and NB600-1291 as you mentioned. For Troponin I: As you mentioned: NB110-2546(Coating)- NB100-73082(Conjugate) and NB100-73087(Coating) - NB110-2546(Conjugate). We do not have any matched pairs for Creatine KinasE MB. We do not have any match pairs for CRP as we have just not tested these in house in ELISA. The customer could use our CRP antibodies in a direct ELISA. We have not had any one use this as an antigen as of yet however if your customer is willing to test this as innovators they are more than welcome. See full details of our Innovators Reward Program here or contact us at innovators@novusbio.com for more information.

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