Troponin I Type 3 (cardiac) Antibody - Azide Free

Novus Biologicals | Catalog # NBP2-20734

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (92%), Canine (95%), Feline (94%), Porcine (93%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all Troponin I/TNNI3 Primary Antibodies »

Product Specifications

Immunogen

Full length human Troponin I Type 3 (cardiac) Recombinant protein.

Reactivity Notes

Cat (94%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody - Azide Free

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734]

Immunohistochemistry-Paraffin: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Troponin I Type 3 (cardiac) antibody detects Troponin I Type 3(cardiac) protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. Green: Troponin I Type 3 (cardiac) stained by Troponin I Type 3 (cardiac) antibody diluted at 1:250. Red: beta Actin, a cytoskeleton marker, stained by beta Actin antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Troponin I Type 3 (cardiac) Antibody

Western Blot: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] -

Western Blot: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Troponin I Type 3 (cardiac) antibody [N1C3] (NBP2-20734) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Troponin I Type 3 (cardiac) Antibody - Azide Free

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] -

Troponin I (cardiac) antibody [N1C3] detects Troponin I (cardiac) protein at cytosol on rat heart by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Troponin I (cardiac) antibody [N1C3] (NBP2-20734) dilution: 1:500.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min
Troponin I Type 3 (cardiac) Antibody - Azide Free

Immunohistochemistry: Troponin I Type 3 (cardiac) Antibody [NBP2-20734] -

Cardiac Troponin I antibody [N1C3] detects Cardiac Troponin I protein at cytosol on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I antibody [N1C3] (NBP2-20734) dilution: 1:500.
Scale bar = M um.
br>Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for Troponin I Type 3 (cardiac) Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Troponin I/TNNI3

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq]

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Gene Symbol

TNNI3

UniProt

P19429

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for Troponin I Type 3 (cardiac) Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Troponin I Type 3 (cardiac) Antibody - Azide Free

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Protocols

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